Fig. 7

Comparison of dCTCF^wt-HA (wt), dCTCF^Δ80–170-HA(Δ80–170) and dCTCF^hN (hN) binding in adult flies. (A) Average signal (RPKM) of ChIP-seq peaks with motif (left) and motif logo (right) for dCTCF protein (anti-dCTCF_C antibodies) in lines expressing dCTCF^wt-HA (wt), dCTCF^Δ80–170-HA (Δ80–170) and CTCF^hN (hN). The motifs associated with the peaks are the same in all three datasets. (B) Average signal (RPKM) (top) and signal heatmaps (bottom) for CP190 in the y¹w¹¹¹⁸ line and dCTCF in the wt-HA, Δ80–170-HA, and hN lines. Different sets of peaks for all three lines (wt-HA, Δ80–170-HA, and hN) are shown (columns): a combined set of dCTCF binding sites with the binding motif overlapping with CP190 binding sites (denoted as “dCTCF motif; CP190 signal”); a combined set of dCTCF binding sites with the motif and not overlapping with CP190 binding sites (denoted as “dCTCF motif; no CP190 signal”); a combined set of dCTCF binding sites without the motif but overlapping with CP190 binding sites (denoted as “no dCTCF motif; CP190 signal”). The “dCTCF motif; CP190 signal” and “dCTCF motif; no CP190 signal” sets were motif-centered, and “no CTCF motif; CP190 signal” set was centered on the peak summit