Fig. 6
From: The N-terminal dimerization domains of human and Drosophila CTCF have similar functionality
Immunoblot analysis of total extract and cytoplasmic, nucleoplasmic, chromatin fractions prepared from two-day-old adult males of y1w1118 (y1w1), dCTCFwt-HA (wt-HA), dCTCFΔ132–170-HA (Δ132–170-HA), dCTCFΔ80–170-HA (Δ80–170-HA), and dCTCFhN (hN) lines. The total extract and cytoplasmic, nucleoplasmic, and chromatin fractions were prepared as described in the Materials and Methods section and briefly explained in the right panel of the figure. Blots were stained with anti-dCTCF_C antibodies and control antibodies against tubulin (cytoplasmic marker), lamin (nuclear marker), and histone H3 (his3, chromatin marker)