Fig. 5
From: The N-terminal dimerization domains of human and Drosophila CTCF have similar functionality
The dimerization domain from hCTCF can functionally replace the DD in dCTCF. (A) Schematic representation of dCTCFwt(wt-HA) and its mutant variants dCTCFΔ80–170-HA (Δ80–170-HA) and dCTCFhN(hN). (B) Morphology of the abdominal segments of the lines with dCTCF mutations. The designations correspond to those shown in Figs. 1 and 2. (C) Localization of dCTCFwt, dCTCFΔ80–170-HA, and dCTCFhN in the polytene chromosomes from third instar female larvae of respective fly lines. The panels show the results of immunostaining of dCTCF variants (with rabbit anti-dCTCF_C antibodies) and CP190 (rat anti-CP190 antibody). DNA was stained with DAPI (blue). Scale bar is 20 μm