Fig. 3

Hmgn knockout cells show increased levels of spontaneous neuronal differentiation. a Immunofluorescence analysis of the neurofilament protein βIII-tubulin (red). Control and Hmgn-knockout cells were fixed 24 h and 48 h after seeding. CON-b cells adhere weakly to slides after 48 h, so no immunofluorescence data are available for this time point. DAPI was used to stain the nuclei (cyan). Scale bar indicates 50 µm. FACS analysis of βIII tubulin expression. b Example dot plots show the distribution of 10,000 cells and the gate that delineates βIII tubulin-positive cells. The percentage of βIII tubulin-positive cells is shown. c Percentage of βIII tubulin-positive cells in each cell line, as determined by FACS (n = 3) (*p < 0.05, **p < 0.001, ***p < 0.0001). E14-NSCs are a negative control. d Relative mRNA expression of the neural-specific markers Nestin, βIII-tubulin and Map2 (no data available for βIII tubulin expression in CON-b cells). The graphs show the fold change relative to parental cells (n = 3–10) (*p < 0.05, **p < 0.01, ***p < 0.001)